Syringe filters with a 22 μm pore size are widely used for pre-filtration and sample clarification in diverse labs. This guide highlights five reliable options that balance sterile and non-sterile needs, membrane chemistries, and membrane diameters to optimize filtration speed and sample integrity. Each product is evaluated on membrane material, pore size, diameter, packaging, and ease of use to help you select the right filter for proteins, buffers, and small-volume samples.
| Product | Membrane | Diameter | Pore Size | Packaging | Type |
|---|---|---|---|---|---|
| KS-Tek Sterile PVDF 25 mm | PVDF | 25 mm | 0.22 μm | 10/pk | Sterile |
| Semmerfeld PES 33 mm Sterile | PES | 33 mm | 0.22 μm | 10/pack | Sterile |
| KS-Tek PTFE 25 mm Hydrophobic | PTFE | 25 mm | 0.22 μm | 25/pk | Non-Sterile |
| Biomed Scientific PVDF 25 mm | PVDF | 25 mm | 0.22 μm | 10/pk | Sterile |
| SimPure PES 33 mm Sterile | PES | 33 mm | 0.22 μm | 10/pk | Sterile |
These selections cover sterile and non-sterile options, with both PES and PVDF membranes and a commonly used 25 mm or 33 mm diameter. When choosing, consider flow rate, protein binding, and the compatibility of the membrane with your solvent system. The following sections provide detailed product insights to help you compare quickly.
KS-Tek Sterile PVDF 25 mm 0.22 μm
Product details: Sterile syringe filters with PVDF membrane, 25 mm diameter, 0.22 μm pore size, packaged 10 per pack. Inlet is female Luer, outlet is male slip Luer. Suitable for sterile filtration of small samples, including buffers and additives. The sterile packaging supports traceability and QC checks in regulated workflows.
Semmerfeld PES 33 mm Sterile 0.22 μm
Product overview: PES membrane with a three-layer design to maximize dirt absorption and flow. Features a female luer lock inlet and male luer slip outlet, 33 mm diameter, 0.22 μm pore size, and sterile packaging. Hydrophilic PES offers good wettability and high flow for buffers, proteins, and reagents, with solid performance in typical lab workflows.
KS-Tek PTFE 25 mm Hydrophobic 0.22 μm
Details: Non-sterile syringe filters with PTFE membranes, 25 mm diameter, 0.22 μm pore size, packed 25 per pack. Hydrophobic PTFE is suitable for separating organic solvents and non-aqueous samples. This option emphasizes chemical compatibility and robust performance in challenging sample matrices, though it lacks sterile packaging.
Biomed Scientific PVDF 25 mm 0.22 μm
Product snapshot: Sterile PVDF syringe filters, 25 mm diameter, 0.22 μm pore size, 10 per pack. Each filter is integrity tested and gamma-irradiated, with an expiry date on each unit to support QC processes. A reliable choice for research applications requiring sterile filtration and traceability.
SimPure PES 33 mm Sterile 0.22 μm
Highlights: PES membrane with 0.22 μm pore size, 33 mm diameter, sterile packaging, and a 10-pack configuration. The product emphasizes higher throughput due to larger surface area and hydrophilicity, suitable for sterile filtrations of small volumes and clean-up steps prior to analytics.
Biomed Scientific PES 25 mm 0.22 μm
Overview: PES membrane filters with 25 mm diameter and 0.22 μm pore size, available in 100/pack or multiple size configurations. The PES material is hydrophilic and suited for protein-containing samples, buffers, and media. Packaging in larger quantities supports high-throughput workflows and bulk testing.
Biomed Scientific PTFE 25 mm 0.22 μm
Product notes: Hydrophobic PTFE membrane with PP housing, non-sterile, 25 mm diameter, 0.22 μm pore size, 100/pack. Inlet is female Luer, outlet is male slip Luer. This option emphasizes chemical compatibility and versatility for challenging solvents, while offering a high-volume pack for busy labs.
Buying Guide
Key factors to consider when choosing a 22 μm syringe filter:
- Membrane material: PVDF offers good chemical resistance and protein compatibility; PES provides hydrophilic flow and high throughput; PTFE is ideal for organic solvents and aggressive chemicals but is commonly non-sterile.
- Sterile vs. non-sterile: For assays requiring contamination-free samples or regulatory compliance, select sterile filters with validated lot testing and expiry dates.
- Diameter and pore size: 25 mm is common for standard syringes; 33 mm increases filtration area for higher throughput. Confirm pore size is 0.22 μm to meet your needs.
- Packaging and quantity: For routine use, smaller packs (10/pk or 25/pk) minimize waste and ensure freshness; high-throughput labs may opt for 100/pack options.
- Connector compatibility: Inlet and outlet fittings (female Luer, male slip Luer) determine compatibility with your syringe setup and downstream equipment.
- Hydrophobic vs. hydrophilic: Hydrophilic PES and PVDF are suitable for aqueous solutions; hydrophobic PTFE is better for organic solvents and ethanol-rich samples, with considerations for potential precipitation.
- QC and traceability: Filtration products with integrity testing, lot certification, and expiry dates improve QA and audit readiness.
- Cost efficiency: Compare total filtration cost per sample, considering membrane life, flow rate, and potential need for re-filtering to maintain sample integrity.
In practice, you may choose a sterile PVDF option for protein-containing samples and a PES or PTFE filter for rapid pre-filtration depending on solvent compatibility and required throughput. Always verify compatibility with your specific assay buffers and analytes to minimize adsorption and loss of material.